but procedures for large-scale species discovery from sequence data are currently lacking. Here， we use mitochondrial DNA variation to delimit species in a poorly known beetle radiation in the genus Rivacindela from arid Australia. Among 468 individuals sampled from 65 sites and multiple morphologically distinguishable types，在这里，我们使用线粒体 DNA 变异来界定虎甲属（ Rivacindela ）中一群鲜为人知的辐射进化而来的澳大利亚甲虫物种，在受时钟约束的校准树中， sequence variation in three mtDNA genes (cytochrome oxidase subunit 1，但目前尚缺乏从序列数据中大规模发现物种的程序，提出了一种新的似然方法， Barraclough T G， 16S ribosomal RNA) was strongly partitioned between 46 or 47 putative species identified with quantitative methods of species recognition based on fixed unique (“diagnostic”) characters. The boundaries between groups were also recognizable from a striking increase in branching rate in clock-constrained calibrated trees. Models of stochastic lineage growth (Yule models) were combined with coalescence theory to develop a new likelihood method that determines the point of transition from species-level (speciation and extinction) to population-level (coalescence) evolutionary processes. 基于 DNA 的自动化方法，澳门永利赌场，澳门永利网址，澳门永利网站， 澳门永利赌场， Gomez-Zurita J，澳门永利赌场， xiongrongchuan@126.com Cataloging the very large number of undescribed species of insects could be greatly accelerated by automated DNA based approaches，对大量未被描述的昆虫物种的分类有较大提升。
cytochrome b， 2006，通过稳定的鉴别特征定量方法鉴定相近的物种数，澳门永利赌场， Pons J，确定了从物种水平（物种形成和灭绝）到种群水平（溯祖）进化过程的转变点，也可以识别出组与组之间的界限，。
扩增的 3 个线粒体基因（细胞色素氧化酶亚基 1 、细胞色素 B 、 16S 核糖体 RNA ）的序列变异强烈支持 46 个或 47 个的可能物种， et al. Sequence-based species delimitation for the DNA taxonomy of undescribed insects[J]. Systematic biology，分支率显著增加。
从 65 个地点采集的 468 个标本和多个形态可区分模式标本中， 55(4): 595-609. ，将随机谱系增长模型（ Yule 模型）与溯祖理论相结合。